GenePix® Pro Acquisition and Analysis Microarray Software from Molecular Devices LLC

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ItemGenePix® Pro Acquisition and Analysis Microarray Software
CompanyMolecular Devices LLC
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Catalog NumberGenePix Pro

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Molecular Devices LLC

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: http://www.moleculardevices.com/

(1) SOD1 is a synthetic lethal target in PPM1D-mutant leukemia cellsbioRxiv : the preprint server for biologyJanuary 17, 2024Zhang L, Hsu JI, Braekeleer ED, Chen CW, Patel TD, Martell AG, Guzman AG, Wohlan K, Waldvogel SM, Urya H, et al.appropriate PMT to obtain optimal signal for this specific set of samples. The images were analyzed with GenePix Pro 7.0 (Molecular Devices, Silicon Valley, CA). Total fluorescence signal intensities of each spot were

(2) Recurring EPHB1 mutations in human cancers alter receptor signalling and compartmentalisation of colorectal cancer cellsCell Commun SignalDecember 15, 2023Snehangshu Kundu, Luís Nunes, Jeremy Adler, Lucy Mathot, Ivaylo Stoimenov, Tobias SjöblomAustria) with constant instrument laser power and PMT settings. Spot segmentation was performed with GenePix Pro 6.0 (Molecular Devices, USA). Acquired raw data were analysed using the linear models for microarray

(3) Distinct patterns of auto-reactive antibodies associated with organ-specific immune-related adverse eventsFront ImmunolDecember 12, 2023Mehmet Altan, Quan-Zhen Li, Qi Wang, Natalie I. Vokes, Ajay Sheshadri, Jianjun Gao, Chengsong Zhu, Hai T. Tran, Saumil Gandhi, Mara B. Antonoff, et al.California, USA) with laser wavelengths of 532 nm and 635 nm. The resulting images were analyzed using Genepix Pro 7.0 software (Molecular Devices). The median signal intensity for each spot was calculated and subtracted

(4) Phosphorylation of MIF by PIP4K2a is necessary for cilia biogenesisCell Death DisDecember 5, 2023Lu Zhang, Hongbing Zhang, Ewud Agborbesong, Julie Xia Zhou, Xiaogang LiGenePix 4000B Microarray Scanner (Molecular Devices, LLC, Sunnyvale, CA), and the probe signals were acquired using GenePix Pro 6.0 software (Molecular Devices). The probes were considered detectable when the signal-to-noise ratios

(5) Changes of serum IgG glycosylation patterns in rheumatoid arthritisClinical ProteomicsDecember 1, 2023Xiaoyue Deng, Xiaomin Liu, Yan Zhang, Dan Ke, Rui Yan, Qian Wang, Xinping Tian, Mengtao Li, Xiaofeng Zeng, Chaojun Huphotomultiplier tube setting of 600. Lectin microarray data analysis For lectin array assays, the GenePix Pro 6.0 software (Molecular Devices, Sunnyvale, CA) and proprietary gal files were used to extract the median

(6) Profiling the autoantibody repertoire reveals autoantibodies associated with mild cognitive impairment and dementiaFront NeurolNovember 30, 2023Hanan Ehtewish, Areej Mesleh, Georgios Ponirakis, Katie Lennard, Hanadi Al Hamad, Mani Chandran, Aijaz Parray, Houari Abdesselem, Patrick Wijten, Julie Decock, et al.aid with image analysis. Automatic extraction and quantification of each spot were performed using GenePix Pro software (Molecular Devices). For each spot, the background signal intensity was calculated using a

(7) IgE and IgG4 epitopes of the peanut allergens shift following oral immunotherapyFrontiers in AllergyNovember 29, 2023Ian M. Rambo, Christina M. Kronfel, Adam R. Rivers, Lauren T. Swientoniewski, Jane K. McBride, Hsiaopo Cheng, Reyna J. Simon, Robert Ryan, Stephen A. Tilles, Jacqueline B. Nesbit, et al.were dried with N2 gas. Washed and dried slides were scanned with a GenePix-4000B scanner (Software: GenePix Pro 7; Molecular Devices, San Jose, CA, USA) to detect IgE binding measured by Cy3-green (532 nm). Slides

(8) Plant-based production of a protective vaccine antigen against the bovine parasitic nematode Ostertagia ostertagiScientific ReportsNovember 22, 2023Laurens Zwanenburg, Jimmy Borloo, Bregt Decorte, Myrna J. M. Bunte, Sanaz Mokhtari, Sonia Serna, Niels-C. Reichardt, Leen J. M. Seys, Angela van Diepen, Arjen Schots, et al.fluorescence index (MFI) was obtained via the G2565BA scanner (Agilent Technologies) and was analysed with GenePix Pro 7.0 software (Molecular Devices). The MFI for each N-glycan spot was exported to Microsoft Excel where

(9) ASPSCR-1 and Sirt-5 alleviate Clonorchis liver fluke rCsNOSIP-induced oxidative stress, proliferation, and migration in cholangiocarcinoma cellsPLOS Neglected Tropical DiseasesNovember 10, 2023Meng Bian, Shan Li, Hanzong Zhou, Lijun Bi, Yong Shen, Chen Tingjin, Xinbing Yu, Yan Huang, Qingxia XuLuxScan 10K-A (CapitalBio Corporation, Beijing, China). The scanned fluorescence data was analyzed with GenePix Pro 6.0 software (Molecular Devices). To generate candidate lists, median normalization was performed for

(10) Retention of Key Characteristics of Unprocessed Chorion Tissue Resulting in a Robust Scaffold to Support Wound HealingInternational Journal of Molecular SciencesOctober 31, 2023Katrina A. Harmon, MaryRose Kammer, Justin T. Avery, Kelly A. Kimmerling, Katie C. Mowryimaged and analyzed to calculate average protein content using a GenePix 4000B microarray scanner and GenePix Pro 7 software, respectively (Molecular Devices, Sunnyvale, CA, USA). Targets present at concentrations

(11) Sialic acid O-acetylation patterns and glycosidic linkage type determination by ion mobility-mass spectrometryNature CommunicationsOctober 25, 2023Gaёl M. Vos, Kevin C. Hooijschuur, Zeshi Li, John Fjeldsted, Christian Klein, Robert P. de Vries, Javier Sastre Toraño, Geert-Jan Boonsusing an Innopsys InnoScan 710 microarray scanner (Carbonne, France). The data were processed with GenePix Pro 7 (Molecular Devices, San Jose, CA) and analyzed with an in-house developed Excel macro using Excel

(12) Tumor-Associated and Systemic Autoimmunity in Pre-Clinical Breast Cancer among Post-Menopausal WomenBiomoleculesOctober 24, 2023Christine G. Parks, Lauren E. Wilson, Michela Capello, Kevin D. Deane, Samir M. HanashImmunoResearch) was performed. Then, the protein arrays were scanned with a GenePix scanner (Molecular Devices GenePix Pro 6.1 4000B, San Jose, CA, USA) using a red laser (635 nm), and local background-subtracted median spot

(13) Features and protective efficacy of human mAbs targeting Mycobacterium tuberculosis arabinomannanJCI InsightOctober 23, 2023Yanyan Liu, Tingting Chen, Yongqi Zhu, Aisha Furey, Todd L. Lowary, John Chan, Stylianos Bournazos, Jeffrey V. Ravetch, Jacqueline M. Achkar4000 Microarray scanner system (Molecular Devices) was used for scanning, and images were analyzed by GenePix Pro 7. The reactivity of mAbs to glycan OS fragments was measured as MFI, which consisted of subtracting

(14) Autoantibody Profiling and Anti-Kinesin Reactivity in ANCA-Associated VasculitisInternational Journal of Molecular SciencesOctober 19, 2023Federica Mescia, Shaghayegh Bayati, Elisabeth Brouwer, Peter Heeringa, Erik J. M. Toonen, Marijke Beenes, Miriam J. Ball, Andrew J. Rees, Renate Kain, Paul A. Lyons, et al.laser scanner (InnoScan® 1100, Innopsys, Chicago, IL, USA), followed by image analysis performed by GenePix Pro 5.1 (Molecular Devices LLC, San Jose, CA, USA). 4.5. Targeted Autoantibody Screening and Anti-Kinesin

(15) High-throughput autoantibody screening identifies differentially abundant autoantibodies in autism spectrum disorderFrontiers in Molecular NeuroscienceOctober 16, 2023Areej Mesleh, Hanan Ehtewish, Katie Lennard, Houari B. Abdesselem, Fouad Al-Shaban, Julie Decock, Nehad M. Alajez, Abdelilah Arredouani, Mohamed M. Emara, Omar Albagha, et al.aid with image analysis. Automatic extraction and quantification of each spot were performed using GenePix Pro 7 software (Molecular Devices), generating the mean foreground and local background pixel intensities

(16) Identification of Anti-SNRPA as a Novel Serological Biomarker for Systemic Sclerosis DiagnosisJournal of Proteome ResearchOctober 6, 2023Chenxi Liu, Guang Song, Songxin Yan, Yangzhige He, Chaojun Hu, Yong Hou, Xiaoting Wen, Liubing Li, Fengchun Zhang, Heng Zhu, et al.scanned with the GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, CA), and analyzed with GenePix Pro 6.0 software (Molecular Devices). 2.4 Fabrication of SSc-Focused Array and Serum Assay In Phase II,

(17) Linear epitope mapping in the E and NS1 proteins of dengue and Zika viruses: Prospection of peptides for vaccines and diagnosticsPLOS ONEOctober 3, 2023Victor Hugo Aquino, Marcilio J. Fumagalli, Angélica Silva, Bento Vidal de Moura Negrini, Alejandra Rojas, Yvalena Guillen, Cynthia Bernal, Luiz Tadeu Moraes FigueiredoMedian fluorescence intensity for each spot, with local background subtraction, was computed using the GenePix Pro 7 (Molecular Devices, USA) or Mapix (Innopsys, France) software. Fluorescence intensity values <1 were

(18) Crossroads between Autoimmunity and COVID-19 in Lung Transplant RecipientsVirusesOctober 3, 2023Madhusudhanan Narasimhan, Alagarraju Muthukumar, Kavithalakshmi Sataranatarajan, Lenin Mahimainathan, Luke Mahan, Irina Timofte, Srinivas Bollineni, John Joerns, Song Zhang, April Gorman, et al.scanned with a Genepix 4400A scanner with laser wavelengths 532 nm for cy3 to generate Tiff images. Genepix Pro 7.0 software was used to analyze the image and generate the Genepix report (GPR) files (Molecular Devices,

(19) Efficacy and safety of immunotherapy combined with single-agent chemotherapy as second- or later-line therapy for metastatic non-small cell lung cancerFrontiers in ImmunologySeptember 18, 2023Dongna Chen, Lin Li, Mingzhao Wang, Xingsheng Hu, Jun Jiang, Weihua Li, Lin Yang, Meng Fan, Yuankai Shi, Fang Lv, et al.(Molecular Devices, CA, USA). The signal intensity of the fluorescent images was extracted using the GenePix Pro image analysis software (Molecular Devices, CA, USA). All antibodies were printed in triplicate, and

(20) Patients with Systemic Juvenile Idiopathic Arthritis (SJIA) Show Differences in Autoantibody Signatures Based on Disease ActivityBiomoleculesSeptember 15, 2023Julie Krainer, Michaela Hendling, Sandra Siebenhandl, Sabrina Fuehner, Christoph Kessel, Emely Verweyen, Klemens Vierlinger, Dirk Foell, Silvia Schönthaler, Andreas Weinhäusel). After microarray processing and scanning, all slides were manually gridded using the Software GenePix® Pro 6.0 (Molecular Devices, Sunnyvale, CA, USA). The resulting median fluorescence intensities per spot

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GenePix® Pro Acquisition and Analysis Microarray Software from Molecular Devices LLC

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GenePix® Pro Acquisition and Analysis Microarray Software from Molecular Devices LLC

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